CE-MS for the Analysis of Amino Acids

  • Karina Trevisan Rodrigues
  • Marina Franco Maggi Tavares
  • Ann Van SchepdaelEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1730)


Amino acids play an important role in clinical analysis. Capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) has proven to possess several characteristics that make it a powerful and useful tool for the analysis of amino acids in clinical studies. Here we present a method for the separation and quantitative analysis of 27 amino acids in urine based on CE-ESI-MS. The method presents an improved resolution between the isomers Leu, Ile, and aIle, in comparison to other CE-ESI-MS methods in the literature. This method is fast, selective, and simple and has improved sensitivity by applying a pH-mediated stacking strategy, showing that it can be successfully used for amino acid analysis and probably for other small cationic metabolites.

Key words

Capillary electrophoresis Mass spectrometry Electrospray ionization Amino acids analysis Targeted metabolomics Urine 


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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Karina Trevisan Rodrigues
    • 1
    • 2
  • Marina Franco Maggi Tavares
    • 1
  • Ann Van Schepdael
    • 2
    Email author
  1. 1.Institute of ChemistryUniversity of Sao Paulo (USP)Sao PauloBrazil
  2. 2.Department of Pharmaceutical and Pharmacological Sciences, Pharmaceutical AnalysisKU Leuven-University of LeuvenLeuvenBelgium

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