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CE-MS for the Analysis of Amino Acids

  • Karina Trevisan Rodrigues
  • Marina Franco Maggi Tavares
  • Ann Van SchepdaelEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1730)

Abstract

Amino acids play an important role in clinical analysis. Capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) has proven to possess several characteristics that make it a powerful and useful tool for the analysis of amino acids in clinical studies. Here we present a method for the separation and quantitative analysis of 27 amino acids in urine based on CE-ESI-MS. The method presents an improved resolution between the isomers Leu, Ile, and aIle, in comparison to other CE-ESI-MS methods in the literature. This method is fast, selective, and simple and has improved sensitivity by applying a pH-mediated stacking strategy, showing that it can be successfully used for amino acid analysis and probably for other small cationic metabolites.

Key words

Capillary electrophoresis Mass spectrometry Electrospray ionization Amino acids analysis Targeted metabolomics Urine 

References

  1. 1.
    Mayboroda OA, Neusüß C, Pelzing M et al (2007) Amino acid profiling in urine by capillary zone electrophoresis–mass spectrometry. J Chromatogr A 1159:149–153. https://doi.org/10.1016/j.chroma.2007.04.055CrossRefPubMedGoogle Scholar
  2. 2.
    Bouatra S, Aziat F, Mandal R et al (2013) The human urine metabolome. PLoS One 8:e73076. https://doi.org/10.1371/journal.pone.0073076CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    De Benedetto GE (2008) Biomedical applications of amino acid detection by capillary electrophoresis. In: Capillary Electrophor. Humana Press, Totowa, NJ, pp 457–481CrossRefGoogle Scholar
  4. 4.
    Wang C, Zhu H, Pi Z et al (2013) Classification of type 2 diabetes rats based on urine amino acids metabolic profiling by liquid chromatography coupled with tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 935:26–31. https://doi.org/10.1016/j.jchromb.2013.07.016CrossRefPubMedGoogle Scholar
  5. 5.
    Sakaguchi Y, Kinumi T, Yamazaki T, Takatsu A (2015) A novel amino acid analysis method using derivatization of multiple functional groups followed by liquid chromatography/tandem mass spectrometry. Analyst 140:1965–1973. https://doi.org/10.1039/C4AN01672FCrossRefPubMedGoogle Scholar
  6. 6.
    Held PK, White L, Pasquali M (2011) Quantitative urine amino acid analysis using liquid chromatography tandem mass spectrometry and aTRAQ reagents. J Chromatogr B Analyt Technol Biomed Life Sci 879:2695–2703. https://doi.org/10.1016/j.jchromb.2011.07.030CrossRefPubMedGoogle Scholar
  7. 7.
    Jabeen R, Payne D, Wiktorowicz J et al (2006) Capillary electrophoresis and the clinical laboratory. Electrophoresis 27:2413–2438. https://doi.org/10.1002/elps.200500948CrossRefPubMedGoogle Scholar
  8. 8.
    Zhong X, Zhang Z, Jiang S, Li L (2014) Recent advances in coupling capillary electrophoresis-based separation techniques to ESI and MALDI-MS. Electrophoresis 35:1214–1225. https://doi.org/10.1002/elps.201300451CrossRefPubMedGoogle Scholar
  9. 9.
    Soga T, Kakazu Y, Robert M et al (2004) Qualitative and quantitative analysis of amino acids by capillary electrophoresis-electrospray ionization-tandem mass spectrometry. Electrophoresis 25:1964–1972. https://doi.org/10.1002/elps.200305791CrossRefPubMedGoogle Scholar
  10. 10.
    Soga T, Heiger DN (2000) Amino acid analysis by capillary electrophoresis electrospray ionization mass spectrometry. Anal Chem 72:1236–1241. https://doi.org/10.1021/ac990976yCrossRefPubMedGoogle Scholar
  11. 11.
    Ramautar R, Mayboroda OA, Derks RJE et al (2008) Capillary electrophoresis time of flight mass spectrometry using noncovalently bilayer coated capillaries for the analysis of amino acids in human urine. Electrophoresis 29:2714–2722. https://doi.org/10.1002/elps.200700929CrossRefPubMedGoogle Scholar
  12. 12.
    Soliman LC, Hui Y, Hewavitharana AK, Chen DDY (2012) Monitoring potential prostate cancer biomarkers in urine by capillary electrophoresis–tandem mass spectrometry. J Chromatogr A 1267:162–169. https://doi.org/10.1016/j.chroma.2012.07.021CrossRefPubMedGoogle Scholar
  13. 13.
    Rodrigues KT, Mekahli D, Tavares MFM, Van Schepdael A (2016) Development and validation of a CE-MS method for the targeted assessment of amino acids in urine. Electrophoresis 37:1039–1047. https://doi.org/10.1002/elps.201500534CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Karina Trevisan Rodrigues
    • 1
    • 2
  • Marina Franco Maggi Tavares
    • 1
  • Ann Van Schepdael
    • 2
    Email author
  1. 1.Institute of ChemistryUniversity of Sao Paulo (USP)Sao PauloBrazil
  2. 2.Department of Pharmaceutical and Pharmacological Sciences, Pharmaceutical AnalysisKU Leuven-University of LeuvenLeuvenBelgium

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