Metabolic Profiling of Urine by Capillary Electrophoresis-Mass Spectrometry Using Non-covalently Coated Capillaries
In the field of metabolomics, capillary electrophoresis-mass spectrometry (CE-MS) can be considered a very useful analytical tool for the profiling of polar and charged metabolites. However, variability of migration time is an important issue in CE. An elegant way to minimize this problem is the use of non-covalently coated capillaries that is dynamic coating of the bare fused-silica capillary with solutions of charged polymers. In this protocol, an improved strategy for the profiling of cationic metabolites in urine by CE-MS using multilayered non-covalent capillary coatings is presented. Capillaries are coated with a bilayer of polybrene (PB) and poly(vinyl sulfonate) (PVS) or with a triple layer of PB, dextran sulfate (DS), and PB. The bilayer- and triple-layer-coated capillaries have a negative and positive outside layer, respectively. It is shown that the use of such capillaries provides very repeatable migration times.
Key wordsCapillary electrophoresis Mass spectrometry Metabolomics Cationic metabolites Non-covalently coated capillaries Urine
Dr. Rawi Ramautar would like to acknowledge the financial support of the Veni and Vidi grant scheme of the Netherlands Organization for Scientific Research (NWO Veni 722.013.008 and Vidi 723.016.003).
- 7.Garcia-Perez I, Whitfield P, Bartlett A, Angulo S, Legido-Quigley C, Hanna-Brown M, Barbas C (2008) Metabolic fingerprinting of Schistosoma Mansoni infection in mice urine with capillary electrophoresis. Electrophoresis 29(15):3201–3206. https://doi.org/10.1002/elps.200800031CrossRefPubMedGoogle Scholar