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GC-MS Analysis of Lipid Oxidation Products in Blood, Urine, and Tissue Samples

  • Anne Barden
  • Trevor A. MoriEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1730)

Abstract

Oxidant stress has been identified as important in the pathology of many diseases. Oxidation products of polyunsaturated fatty acids collectively termed isoprostanes, neuroprostanes, and isofurans are considered the most reliable measures of in vivo lipid oxidation, and they are widely used to assess oxidant stress in various diseases. Here we describe the measurement of these lipid oxidation products using gas chromatography mass spectrometry with electron capture negative ionization.

Key words

Lipid oxidation F2-isoprostanes Neuroprostanes Isofurans Gas chromatography mass spectrometry 

Notes

Acknowledgments

This work was in part funded by the National Health and Medical Research Council of Australia, the National Heart Foundation of Australia, and the Royal Perth Hospital Medical Research Foundation.

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Medical School, Royal Perth Hospital UnitUniversity of Western AustraliaPerthAustralia

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