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Analysis of Oxysterols

  • Fabien Riols
  • Justine Bertrand-MichelEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1730)

Abstract

Oxysterols are oxygenated derivatives of cholesterol formed in the human body or ingested in the diet. By modulating the activity of many proteins (for instance, liver X receptors, oxysterol-binding proteins, some ATP-binding cassette transporters), oxysterols can affect many cellular functions and influence various physiological processes (e.g., cholesterol metabolism, membrane fluidity regulation, intracellular signaling pathways). Due to their crucial role, it is important to be able to quantify them in pathological conditions. The method described here permits to measure the content of oxysterol in plasma, cell, or media using GC-MS.

Key words

Oxysterol Plasma Gas chromatography mass spectrometry 

Notes

Acknowledgments

This work was in part funded by National Health and Medical Research Council of France (Inserm) and the French National Infrastructure MetaboHUB-ANR-11-INBS-0010.

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.MetaToul-Lipidomic MetaboHUB Core FacilityInserm U1048Toulouse Cedex 4France

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