Analysis of Oxysterols

  • Fabien Riols
  • Justine Bertrand-MichelEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1730)


Oxysterols are oxygenated derivatives of cholesterol formed in the human body or ingested in the diet. By modulating the activity of many proteins (for instance, liver X receptors, oxysterol-binding proteins, some ATP-binding cassette transporters), oxysterols can affect many cellular functions and influence various physiological processes (e.g., cholesterol metabolism, membrane fluidity regulation, intracellular signaling pathways). Due to their crucial role, it is important to be able to quantify them in pathological conditions. The method described here permits to measure the content of oxysterol in plasma, cell, or media using GC-MS.

Key words

Oxysterol Plasma Gas chromatography mass spectrometry 



This work was in part funded by National Health and Medical Research Council of France (Inserm) and the French National Infrastructure MetaboHUB-ANR-11-INBS-0010.


  1. 1.
    Russell DW (2003) The enzymes, regulation, and genetics of bile acid synthesis. Annu Rev Biochem 72:137–174. Scholar
  2. 2.
    Iuliano L (2011) Pathways of cholesterol oxidation via non-enzymatic mechanisms. Chem Phys Lipids 164(6):457–468. Scholar
  3. 3.
    Bauman DR, Bitmansour AD, McDonald JG, Thompson BM, Liang G, Russell DW (2009) 25-hydroxycholesterol secreted by macrophages in response to toll-like receptor activation suppresses immunoglobulin a production. Proc Natl Acad Sci U S A 106(39):16764–16769. Scholar
  4. 4.
    Hannedouche S, Zhang J, Yi T, Shen W, Nguyen D, Pereira JP, Guerini D, Baumgarten BU, Roggo S, Wen B, Knochenmuss R, Noel S, Gessier F, Kelly LM, Vanek M, Laurent S, Preuss I, Miault C, Christen I, Karuna R, Li W, Koo DI, Suply T, Schmedt C, Peters EC, Falchetto R, Katopodis A, Spanka C, Roy MO, Detheux M, Chen YA, Schultz PG, Cho CY, Seuwen K, Cyster JG, Sailer AW (2011) Oxysterols direct immune cell migration via EBI2. Nature 475(7357):524–527. Scholar
  5. 5.
    Kotti TJ, Ramirez DM, Pfeiffer BE, Huber KM, Russell DW (2006) Brain cholesterol turnover required for geranylgeraniol production and learning in mice. Proc Natl Acad Sci U S A 103(10):3869–3874. Scholar
  6. 6.
    Suzuki R, Lee K, Jing E, Biddinger SB, McDonald JG, Montine TJ, Craft S, Kahn CR (2010) Diabetes and insulin in regulation of brain cholesterol metabolism. Cell Metab 12(6):567–579. Scholar
  7. 7.
    Porter FD, Scherrer DE, Lanier MH, Langmade SJ, Molugu V, Gale SE, Olzeski D, Sidhu R, Dietzen DJ, Fu R, Wassif CA, Yanjanin NM, Marso SP, House J, Vite C, Schaffer JE, Ory DS (2010) Cholesterol oxidation products are sensitive and specific blood-based biomarkers for Niemann-pick C1 disease. Sci Transl Med 2(56):56ra81. Scholar
  8. 8.
    Griffiths WJ, Wang Y, Karu K, Samuel E, McDonnell S, Hornshaw M, Shackleton C (2008) Potential of sterol analysis by liquid chromatography-tandem mass spectrometry for the prenatal diagnosis of Smith-Lemli-Opitz syndrome. Clin Chem 54(8):1317–1324. Scholar
  9. 9.
    Matysik S, Klunemann HH, Schmitz G (2012) Gas chromatography-tandem mass spectrometry method for the simultaneous determination of oxysterols, plant sterols, and cholesterol precursors. Clin Chem 58(11):1557–1564. Scholar
  10. 10.
    Saeed A, Floris F, Andersson U, Pikuleva I, Lovgren-Sandblom A, Bjerke M, Paucar M, Wallin A, Svenningsson P, Bjorkhem I (2014) 7alpha-hydroxy-3-oxo-4-cholestenoic acid in cerebrospinal fluid reflects the integrity of the blood-brain barrier. J Lipid Res 55(2):313–318. Scholar

Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.MetaToul-Lipidomic MetaboHUB Core FacilityInserm U1048Toulouse Cedex 4France

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