GC-MS Analysis of Short-Chain Fatty Acids in Feces, Cecum Content, and Blood Samples

  • Lisa R. Hoving
  • Marieke Heijink
  • Vanessa van Harmelen
  • Ko Willems van Dijk
  • Martin GieraEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1730)


Short-chain fatty acids, the end products of fermentation of dietary fibers by the gut microbiota, have been shown to exert multiple effects on mammalian metabolism. For the analysis of short-chain fatty acids, gas chromatography–mass spectrometry is a very powerful and reliable method. Here, we describe a fast, reliable, and reproducible method for the separation and quantification of short-chain fatty acids in mouse feces, cecum content, and blood samples (i.e., plasma or serum) using gas chromatography–mass spectrometry. The short-chain fatty acids analyzed include acetic acid, propionic acid, butyric acid, valeric acid, hexanoic acid, and heptanoic acid.

Key words

Short-chain fatty acids Gas chromatography–mass spectrometry GC-MS Targeted metabolomics PFBBr 


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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Lisa R. Hoving
    • 1
  • Marieke Heijink
    • 2
  • Vanessa van Harmelen
    • 1
  • Ko Willems van Dijk
    • 1
    • 3
  • Martin Giera
    • 2
    Email author
  1. 1.Einthoven Laboratory for Experimental Vascular Medicine, Department of Human GeneticsLeiden University Medical Center (LUMC)LeidenThe Netherlands
  2. 2.Center for Proteomics and MetabolomicsLeiden University Medical Center (LUMC)LeidenThe Netherlands
  3. 3.Division of Endocrinology, Department of MedicineLeiden University Medical Center (LUMC)LeidenThe Netherlands

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