Direct Infusion-Tandem Mass Spectrometry (DI-MS/MS) Analysis of Complex Lipids in Human Plasma and Serum Using the Lipidyzer™ Platform
Lipids play a key role in the signaling pathways of cancer, cardiovascular, diabetic, and inflammatory diseases. A major challenge in the analysis of lipids is the many isobaric interferences present in highly complex samples that confound identification and accurate quantitation. After obtaining the total lipid extract from a sample, differential mobility separation has proven to be a powerful tool for gas-phase fractionation of lipid classes. When combined with mass spectrometry, this allows the unambiguous identification and thus quantification of lipid molecular species. These components, sample extraction, gas-phase separation, and mass spectrometry, form the basis of a novel integrated quantitative lipid analysis platform.
Key wordsDI-MS/MS Lipidyzer Lipidomics Plasma Serum
The author would like to thank the Lipidyzer development team at Metabolon (Raleigh, NC) and the Lipidyzer development team at SCIEX (Framingham, MA) including Avanti Polar Lipids (Alabaster, AL) for manufacturing the novel internal standards accompanying the Lipidyzer Platform.
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