Chemical Isotope Labeling LC-MS for Human Blood Metabolome Analysis
Blood is a widely used biofluid in discovery metabolomic research to search for clinical metabolite biomarkers of diseases. Analyzing the entire human blood metabolome is a major analytical challenge, as blood, after being processed into serum or plasma, contains thousands of metabolites with diverse chemical and physical properties as well as a wide range of concentrations. We describe an enabling method based on high-performance chemical isotope labeling (CIL) liquid chromatography-mass spectrometry (LC-MS) for in-depth quantification of the metabolomic differences in comparative blood samples with high accuracy and precision.
Key wordsChemical isotope labeling Dansylation DmPA LC-MS Metabolomics Blood
This work was supported by Genome Canada, the Natural Sciences and Engineering Research Council of Canada (NSERC), and Canada Research Chairs (CRC) programs.
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