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Chemical Isotope Labeling LC-MS for Human Blood Metabolome Analysis

  • Wei Han
  • Liang LiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1730)

Abstract

Blood is a widely used biofluid in discovery metabolomic research to search for clinical metabolite biomarkers of diseases. Analyzing the entire human blood metabolome is a major analytical challenge, as blood, after being processed into serum or plasma, contains thousands of metabolites with diverse chemical and physical properties as well as a wide range of concentrations. We describe an enabling method based on high-performance chemical isotope labeling (CIL) liquid chromatography-mass spectrometry (LC-MS) for in-depth quantification of the metabolomic differences in comparative blood samples with high accuracy and precision.

Key words

Chemical isotope labeling Dansylation DmPA LC-MS Metabolomics Blood 

Notes

Acknowledgment

This work was supported by Genome Canada, the Natural Sciences and Engineering Research Council of Canada (NSERC), and Canada Research Chairs (CRC) programs.

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Department of ChemistryUniversity of AlbertaEdmontonCanada

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