Establishing and Performing Targeted Multi-residue Analysis for Lipid Mediators and Fatty Acids in Small Clinical Plasma Samples
LC-MS/MS- and GC-MS-based targeted metabolomics is typically conducted by analyzing and quantifying a cascade of metabolites with methods specifically developed for the metabolite class. Here we describe an approach for the development of multi-residue analytical profiles, calibration standards, and internal standard solutions in support of a fast, simple, and low-cost plasma sample preparation that captures and quantitates a range of metabolite cascades.
Key wordsOxylipins Endocannabinoids Bile acids Nonesterified fatty acids Targeted metabolomics LC-MS/MS Metabolic profiling
The authors would like to thank Ira J. Gray, Michael R. La Frano, and William R. Keyes for their technical support on the development and implementation of these assays over the past 10 years. The methods reported in this chapter were developed as part of research projects funded by the United States Department of Agriculture (5306-51530-016-00D, 2032-51530-019-00-D, 2032-51530-022-00-D) and DK/NIDDK NIH HHS Grant U24 DK097154/United States
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