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Mutational Analysis of Binding Protein–Chemoreceptor Interactions

  • Andrew L. Seely
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1729)

Abstract

Interactions between ligands and chemoreceptors in Escherichia coli and Salmonella enterica can be studied through genetic manipulation of the actors involved. Sequence analysis and modeling can reveal potential sites of interaction, and these sites can be deleted or mutated and the effects tested through various in vivo chemotaxis assays to ascertain their importance during interaction. Here, the approach for analysis of the interaction between a major E. coli chemoreceptor and its binding protein ligand is described.

Keywords

Site-directed mutagenesis Alanine-scanning mutagenesis Chemoreceptor Binding protein Polymerase chain reaction Pfu Ultra Chemotaxis Transformation 

Notes

Acknowledgment

I would like to acknowledge the guidance of Dr. Michael Manson over the course of my research on bacterial chemotaxis. I would also like to acknowledge the student and postdoctoral members of the Manson Lab at Texas A&M University for their hard work and collaboration with these research endeavors.

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Division of Math and ScienceUniversity of Arkansas Community College BatesvilleBatesvilleUSA

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