Abstract
The combination of genetic code expansion (GCE) and baculovirus-based protein expression in Spodoptera frugiperda cells is a powerful tool to express multiprotein complexes with site-specifically introduced noncanonical amino acids. This protocol describes the integration of synthetase and tRNA gene indispensable for GCE into the backbone of the Bacmid, the Tn7-mediated transposition of various genes of interest, as well as the final expression of protein using the MultiBacTAG system with different noncanonical amino acids.
Key words
- MultiBac
- Baculovirus
- Genetic code expansion
- Noncanonical amino acids
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Koehler, C., Lemke, E.A. (2018). MultiBacTAG-Genetic Code Expansion Using the Baculovirus Expression System in Sf21 Cells. In: Lemke, E. (eds) Noncanonical Amino Acids. Methods in Molecular Biology, vol 1728. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7574-7_19
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DOI: https://doi.org/10.1007/978-1-4939-7574-7_19
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