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Neurotrophic Factors pp 49–61Cite as

Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from the Cortex, Spinal Cord, and Cerebellum

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1727))

Abstract

The protocol described in this chapter covers the preparation and culture of enriched populations of microglia, astrocytes, and oligodendrocytes from the cortex and spinal cord of neonatal rat and mouse. The procedure is based on enzymatic digestion of the tissue, followed by the culture of a mixed glial cell population which is then utilized as the starting point for the isolation, via differential attachment, of the different cell types.

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Authors and Affiliations

  1. Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Padua, Italy

    Stephen D. Skaper & Laura Facci

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  1. Stephen D. Skaper
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  2. Laura Facci
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Corresponding author

Correspondence to Stephen D. Skaper .

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Editors and Affiliations

  1. Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy

    Stephen D. Skaper

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Skaper, S.D., Facci, L. (2018). Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from the Cortex, Spinal Cord, and Cerebellum. In: Skaper, S. (eds) Neurotrophic Factors. Methods in Molecular Biology, vol 1727. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7571-6_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7571-6_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7570-9

  • Online ISBN: 978-1-4939-7571-6

  • eBook Packages: Springer Protocols

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