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Laser Capture Microdissection pp 273–284Cite as

Immuno-Laser-Capture Microdissection for the Isolation of Enriched Glial Populations from Frozen Post-Mortem Human Brain

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1723))

Abstract

Laser-capture microdissection (LCM) has revolutionized the isolation of defined regions and specific cell populations from human tissue. This approach used in combination with immunohistochemistry (immuno-LCM) has become a valuable method for isolating individual cell-types from a complex heterogeneous population. Here, we describe the detailed methodology required for the isolation of enriched populations of GFAP+ astrocytes, OSP+ oligodendrocytes, and CD68+ microglia from frozen post-mortem human central nervous system tissue using immuno-LCM.

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Authors and Affiliations

  1. Sheffield Institute for Translational Neuroscience, University of Sheffield, Sheffield, UK

    Julie E. Simpson, Stephen B. Wharton & Paul R. Heath

Authors
  1. Julie E. Simpson
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  2. Stephen B. Wharton
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  3. Paul R. Heath
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Corresponding author

Correspondence to Stephen B. Wharton .

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Editors and Affiliations

  1. School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, United Kingdom

    Graeme I. Murray

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© 2018 Springer Science+Business Media, LLC

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Simpson, J.E., Wharton, S.B., Heath, P.R. (2018). Immuno-Laser-Capture Microdissection for the Isolation of Enriched Glial Populations from Frozen Post-Mortem Human Brain. In: Murray, G. (eds) Laser Capture Microdissection. Methods in Molecular Biology, vol 1723. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7558-7_16

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  • DOI: https://doi.org/10.1007/978-1-4939-7558-7_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7557-0

  • Online ISBN: 978-1-4939-7558-7

  • eBook Packages: Springer Protocols

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