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The No-Nonsens SDS-PAGE

  • Lars BackmanEmail author
  • Karina Persson
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1721)

Abstract

The discontinuous polyacrylamide gel electrophoresis system devised by Laemmli (Nature 227:680–685, 1970) has not only been used in numerous laboratories but has also been modified in several ways since its birth. In our laboratories, we use a modified Laemmli SDS-PAGE system for following protein purification as well as for analysis of certain protein-protein interactions, mainly involving filametous actin.

Key words

SDS-PAGE polyacrylamide gel electrophoresis 

References

  1. 1.
    Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685CrossRefPubMedGoogle Scholar
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    Addario B, Sandblad L, Persson K, Backman L (2016) Characterisation of Schizosaccharomyces pombe alpha-actinin. PeerJ 4:e1858CrossRefPubMedPubMedCentralGoogle Scholar
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    Schägger H, von Jagow G (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166:368–379CrossRefPubMedGoogle Scholar
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    Schägger H (2006) Tricine-SDS-PAGE. Nat Protoc 1:16–22CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Department of ChemistryUmeå UniversitySE-901 87 UmeåSweden

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