The No-Nonsens SDS-PAGE

  • Lars BackmanEmail author
  • Karina Persson
Part of the Methods in Molecular Biology book series (MIMB, volume 1721)


The discontinuous polyacrylamide gel electrophoresis system devised by Laemmli (Nature 227:680–685, 1970) has not only been used in numerous laboratories but has also been modified in several ways since its birth. In our laboratories, we use a modified Laemmli SDS-PAGE system for following protein purification as well as for analysis of certain protein-protein interactions, mainly involving filametous actin.

Key words

SDS-PAGE polyacrylamide gel electrophoresis 


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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Department of ChemistryUmeå UniversitySE-901 87 UmeåSweden

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