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Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo

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Schizosaccharomyces pombe

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1721))

Abstract

This chapter describes a microfluidic device that enables immobilization and culturing of single rod-shaped S. pombe cells in a stand-up mode. The wide-band electrical impedance spectroscopy (EIS) has been integrated in the microfluidic device to continuously measure cell growth of single S. pombe cells. Cell growth curves showing cellular and intracellular features at high spatiotemporal resolution can be obtained from EIS signals. The features include longitudinal cell elongation in the G2 phase, mitosis, and cell division during an entire cell cycle of S. pombe cells. Microfluidics-based EIS systems provide, hence, a tool for dynamic single-cell studies.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 61404027 and No. 61774036), the National Key Basic Research Program of China (No. 2015CB352100), and the Swiss SystemsX.ch program within the RTD project “CINA.”

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Authors and Affiliations

  1. Southeast University, Key Laboratory of MEMS of Ministry of Education, Nanjing, China

    Zhen Zhu

  2. Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland

    Olivier Frey & Andreas Hierlemann

Authors
  1. Zhen Zhu
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  2. Olivier Frey
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  3. Andreas Hierlemann
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Corresponding author

Correspondence to Zhen Zhu .

Editor information

Editors and Affiliations

  1. Department of Biological Sciences, Winston-Salem State University, Winston-Salem, North Carolina, USA

    Teresa L. Singleton

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Zhu, Z., Frey, O., Hierlemann, A. (2018). Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo. In: Singleton, T. (eds) Schizosaccharomyces pombe. Methods in Molecular Biology, vol 1721. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7546-4_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7546-4_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7545-7

  • Online ISBN: 978-1-4939-7546-4

  • eBook Packages: Springer Protocols

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