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Preparation of Solutions and Reagents

  • Teresa L. SingletonEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1721)

Abstract

The ability to prepare accurate solutions, reagents, and buffers will determine the outcome of a successful experiment. Much time and effort spent in a lab will consist of making solutions, reagents, and buffers. This chapter introduces and refreshes the skill of making solutions including: molarity (M) concentration, percentage (%) concentration, mass/volume concentrations, X-factor concentration, working concentrations from stock solutions, conversions from percentage (%) to molarity (M) concentration, and conversions from molarity (M) to percentage (%) concentration. Basic formulas and examples for how to make solution concentrations are covered.

Key words

Molarity Solutions Buffers Dilutions Media X-factor concentration 

Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Department of Biological ScienceWinston-Salem State UniversityWinston-SalemUSA

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