Preparation of Solutions and Reagents

  • Teresa L. SingletonEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1721)


The ability to prepare accurate solutions, reagents, and buffers will determine the outcome of a successful experiment. Much time and effort spent in a lab will consist of making solutions, reagents, and buffers. This chapter introduces and refreshes the skill of making solutions including: molarity (M) concentration, percentage (%) concentration, mass/volume concentrations, X-factor concentration, working concentrations from stock solutions, conversions from percentage (%) to molarity (M) concentration, and conversions from molarity (M) to percentage (%) concentration. Basic formulas and examples for how to make solution concentrations are covered.

Key words

Molarity Solutions Buffers Dilutions Media X-factor concentration 

Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Department of Biological ScienceWinston-Salem State UniversityWinston-SalemUSA

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