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Peptidomics pp 349-358 | Cite as

Analysis of the Snake Venom Peptidome

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1719)

Abstract

Snake venom peptidomes are known to be a large source of molecules with different pharmacological properties. The complexity and variability of snake venoms, the presence of proteinases, and the lack of complete species-specific genome sequences make snake venom peptidome profiling a challenging task that requires especial technical strategies for sample processing and mass spectrometric analysis. Here we describe a method for assessing the content of snake venom peptides and highlight the importance of sampling procedures, as they substantially influence the peptidomic complexity of snake venoms.

Key words

Snake venom Peptidome Solid-phase extraction Proteinase inhibition Mass spectrometry 
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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Laboratório Especial de Toxinologia Aplicada, Center of Toxins, Immune-Response and Cell Signaling (CeTICS)Instituto ButantanSão PauloBrazil
  2. 2.Instituto de Ciência e TecnologiaUniversidade Federal de São PauloSão José dos CamposBrazil
  3. 3.Departamento de Bioquímica, Escola Paulista de MedicinaUniversidade Federal de São PauloSão PauloBrazil

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