Modeling Transient Focal Ischemic Stroke in Rodents by Intraluminal Filament Method of Middle Cerebral Artery Occlusion

  • Mary Susan Lopez
  • Raghu VemugantiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1717)


The middle cerebral artery occlusion (MCAO) model is widely used for inducing a focal cerebral ischemic insult (stroke) in rodents. Here, we describe a method for transient MCAO technique without craniotomy in both mice and rats. In our laboratory, this technique yields consistent secondary brain damage that evolves over a period of 3–7 days of reperfusion after transient MCAO. We also describe the methods for analyzing postischemic motor dysfunction and infarct volume in rodents subjected to transient MCAO.

Key words

Middle cerebral artery occlusion Focal cerebral ischemia Transcardiac perfusion Beam-walk test Rotarod test Sticker removal test Infarct volume 


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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Department of NeurosurgeryUniversity of Wisconsin-MadisonMadisonUSA

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