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A Rat Burn Injury Model for Studying Changes in Microvascular Permeability

  • Katie Wiggins-Dohlvik
  • Binu TharakanEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1717)

Abstract

The management of burn patients is an extremely complex and clinically challenging for patient care. Aside from the increasing reports of burn injury and morbidity and mortality directly related to it, the pathobiology of burn trauma is not clearly understood. The rat model of burn trauma described here is currently used in research laboratories to study various aspects of burn injury, including vascular dysfunctions. This model demonstrates the infliction of thermal injury in Sprague-Dawley rats using a well-established boiled water approach. We have utilized intravital microscopy to examine the microvascular hyperpermeability, the excessive leakage of proteins and fluids from the intravascular space to the extravascular space in mesenteric postcapillary venules using this model. An increase in microvascular permeability is a strong indicator of microvascular dysfunctions leading to tissue edema in burn trauma.

Key words

Burn injury Thermal injury Burn trauma Vascular hyperpermeability 

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Department of SurgeryTexas A&M University Health Science Center, College of Medicine, Baylor Scott and White Research InstituteTempleUSA

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