A Rat Burn Injury Model for Studying Changes in Microvascular Permeability

  • Katie Wiggins-Dohlvik
  • Binu TharakanEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1717)


The management of burn patients is an extremely complex and clinically challenging for patient care. Aside from the increasing reports of burn injury and morbidity and mortality directly related to it, the pathobiology of burn trauma is not clearly understood. The rat model of burn trauma described here is currently used in research laboratories to study various aspects of burn injury, including vascular dysfunctions. This model demonstrates the infliction of thermal injury in Sprague-Dawley rats using a well-established boiled water approach. We have utilized intravital microscopy to examine the microvascular hyperpermeability, the excessive leakage of proteins and fluids from the intravascular space to the extravascular space in mesenteric postcapillary venules using this model. An increase in microvascular permeability is a strong indicator of microvascular dysfunctions leading to tissue edema in burn trauma.

Key words

Burn injury Thermal injury Burn trauma Vascular hyperpermeability 


  1. 1.
    Alvarado R, Chung KK, Cancio LC, Wolf SE (2009) Burn resuscitation. Burns 35:4–14CrossRefPubMedGoogle Scholar
  2. 2.
    Haberal M, Abali AE, Karakayali H (2010) Fluid management in major burn injuries. Indian J Plast Surg 43(Suppl):S29–S36CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Dries DJ (2009) Management of burn injuries – recent developments in resuscitation, infection control and outcomes research. Scand J Trauma Resusc Emerg Med 17(14):1–13Google Scholar
  4. 4.
    Greenhalgh DG (2007) Burn resuscitation. J Burn Care Res 28:555–565CrossRefPubMedGoogle Scholar
  5. 5.
    Pham TN, Cancio LC, Gibran NS (2008) American Burn Association practice guidelines burn shock resuscitation. J Burn Care Res 29:257–266CrossRefPubMedGoogle Scholar
  6. 6.
    Demling RH (2005) The burn Edema process: current concepts. J Burn Care Rehabil 26:207–227CrossRefPubMedGoogle Scholar
  7. 7.
    Walker H, Mason A (1968) A standard animal burn. J Trauma 8:1049–1051CrossRefPubMedGoogle Scholar
  8. 8.
    Stagg HW, Whaley JG, Tharakan B, Hunter FA, Jupiter D, Little DC, Davis ML, Smythe WR, Childs EW (2013) Doxycycline attenuates burn-induced microvascular hyperpermeability. J Trauma Acute Care Surg 75:1040–1046CrossRefPubMedGoogle Scholar
  9. 9.
    Wiggins-Dohlvik K, Oakley RP, Han MS, Stagg HW, Alluri H, Shaji CA, Davis ML, Tharakan B (2016) Tissue inhibitor of metalloproteinase-2 inhibits burn-induced derangements and hyperpermeability in microvascular endothelial cells. Am J Surg 211:197–205CrossRefPubMedGoogle Scholar
  10. 10.
    Wiggins-Dohlvik K, Han MS, Stagg HW, Alluri H, Shaji CA, Oakley RP, Davis ML, Tharakan B (2014) Melatonin inhibits thermal injury-induced hyperpermeability in microvascular endothelial cells. J Trauma Acute Care Surg 77:899–905CrossRefPubMedGoogle Scholar
  11. 11.
    Wiggins-Dohlvik K, Stagg HW, Han MS, Alluri H, Oakley RP, Anasooya Shaji C, Davis ML, Tharakan B (2016) Doxycycline attenuates lipopolysaccharide-induced microvascular endothelial cell derangements. Shock 45:626–633CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Department of SurgeryTexas A&M University Health Science Center, College of Medicine, Baylor Scott and White Research InstituteTempleUSA

Personalised recommendations