Advertisement

Measurement of Intracranial Pressure in Freely Moving Rats

  • Michael R. Williamson
  • Roseleen F. John
  • Frederick ColbourneEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1717)

Abstract

Brain injury, such as from stroke and trauma, can be complicated by elevated intracranial pressure (ICP). Although raised ICP can be a significant determinant of morbidity and mortality, clinical studies often report widely varying ICP measurements depending on location of measurement and technique used. For the same reasons, reported ICP measurements also vary widely in animal models. The need for anesthesia or tethered connections with some methods of ICP measurement in animals may introduce additional confounds. Moreover, these methods are not well suited for prolonged, continuous measurement. Here, we describe an approach to continually measure ICP in awake, freely moving rats for several days. This technique uses a commercially available, wireless pressure sensor mounted on the head to measure ICP from the epidural space via a fluid-filled catheter. We have demonstrated that this approach reliably detects elevations in ICP that last for several days after ischemic and hemorrhagic strokes in rat.

Key words

Stroke Trauma ICP Intracranial pressure Telemetry Rodent Animal model Blood pressure sensor Brain injury 

References

  1. 1.
    Steiner T, Weber R, Krieger D (2001) Increased intracerebral pressure following stroke. Curr Treat Options Neurol 3:441–450CrossRefPubMedGoogle Scholar
  2. 2.
    Steiner LA, Andrews PJD (2006) Monitoring the injured brain. ICP and CBF. Br J Anaesth 97:26–38CrossRefPubMedGoogle Scholar
  3. 3.
    Sykora M, Steinmacher S, Steiner T et al (2014) Association of intracranial pressure with outcome in comatose patients with intracerebral hemorrhage. J Neurol Sci 342:141–145CrossRefPubMedGoogle Scholar
  4. 4.
    Kirkman MA, Smith M (2014) Intracranial pressure monitoring, cerebral perfusion pressure estimation, and ICP/CPP-guided therapy: a standard of care or optional extra after brain injury? Br J Anaesth 112:35–46CrossRefPubMedGoogle Scholar
  5. 5.
    Uldall M, Juhler M, Skjolding AD et al (2014) A novel method for long-term monitoring of intracranial pressure in rats. J Neurosci Methods 227:1–9CrossRefPubMedGoogle Scholar
  6. 6.
    Hiploylee C, Colbourne F (2014) Intracranial pressure measured in freely moving rats for days after intracerebral hemorrhage. Exp Neurol 255:49–55CrossRefPubMedGoogle Scholar
  7. 7.
    Flower O, Hellings S (2012) Sedation in traumatic brain injury. Emerg Med Int 2012:1–11CrossRefGoogle Scholar
  8. 8.
    Schifilliti D, Grasso G, Conti A, Fodale V (2010) Anaesthetic-related neuroprotection: intravenous or inhalational agents? CNS Drugs 24:893–907PubMedGoogle Scholar
  9. 9.
    Chowdhury UR, Holman BH, Fautsch MP (2013) A novel rat model to study the role of intracranial pressure modulation on optic neuropathies. PLoS One 8:1–8Google Scholar
  10. 10.
    Zwienenberg M, Gong QZ, Lee LL et al (1999) ICP monitoring in the rat: comparison of monitoring in the ventricle, brain parenchyma, and cisterna magna. J Neurotrauma 16:1095–1102CrossRefPubMedGoogle Scholar
  11. 11.
    Silasi G, MacLellan CL, Colbourne F (2009) Use of telemetry blood pressure transmitters to measure intracranial pressure (ICP) in freely moving rats. Curr Neurovasc Res 6:62–69CrossRefPubMedGoogle Scholar
  12. 12.
    Poca MA, Sahuquillo J, Topczewski T et al (2007) Is intracranial pressure monitoring in the epidural space reliable? Fact and fiction. J Neurosurg 106:548–556CrossRefPubMedGoogle Scholar
  13. 13.
    MacLellan CL, Silasi G, Poon CC et al (2008) Intracerebral hemorrhage models in rat: comparing collagenase to blood infusion. J Cereb Blood Flow Metab 28:516–525CrossRefPubMedGoogle Scholar
  14. 14.
    Olson DM, Batjer HH, Abdulkadir K, Hall CE (2013) Measuring and monitoring ICP in neurocritical care, results from a national practice survey. Neurocrit Care 20:15–20CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Michael R. Williamson
    • 1
  • Roseleen F. John
    • 1
  • Frederick Colbourne
    • 2
    Email author
  1. 1.Neuroscience and Mental Health InstituteUniversity of AlbertaEdmontonCanada
  2. 2.Department of Psychology, Neuroscience and Mental Health InstituteUniversity of AlbertaEdmontonCanada

Personalised recommendations