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Traumatic and Ischemic Injury pp 189–206Cite as

Methods to Study the Innate Immune Response to Sepsis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1717))

Abstract

This chapter describes techniques to measure the innate immune response in the mouse cecal ligation and puncture model of sepsis. The reader will learn how to perform retro-orbital bleeds to harvest serum from mice and learn how to perform peritoneal lavage to harvest cells and inflammatory mediators from this compartment. The enzyme-linked immunosorbent assay (ELISA) technique is described as a method to measure the levels of cytokines and chemokines in these fluids. Additionally, this chapter describes techniques to stain the cellular fraction of the peritoneal lavage with fluorescently labeled antibodies, and perform fluorescence activated cell sorting (FACS) to quantify macrophages and neutrophils in this compartment.

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Author information

Authors and Affiliations

  1. Center of Emphasis in Infectious Diseases, Department of Biomedical Sciences, Texas Tech University Health Sciences Center El Paso, El Paso, TX, USA

    Wendy E. Walker

Authors
  1. Wendy E. Walker
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Corresponding author

Correspondence to Wendy E. Walker .

Editor information

Editors and Affiliations

  1. Department of Surgery, Texas A&M University Health Science Center, College of Medicine, Baylor Scott and White Research Institute, Temple, Texas, USA

    Binu Tharakan

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Walker, W.E. (2018). Methods to Study the Innate Immune Response to Sepsis. In: Tharakan, B. (eds) Traumatic and Ischemic Injury. Methods in Molecular Biology, vol 1717. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7526-6_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7526-6_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7524-2

  • Online ISBN: 978-1-4939-7526-6

  • eBook Packages: Springer Protocols

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