A Murine Model of Myocardial Ischemia–Reperfusion Injury

  • Zhaobin Xu
  • Kevin E. McElhanon
  • Eric X. Beck
  • Noah WeislederEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1717)


Ligation of the left anterior descending (LAD) coronary artery in the mouse heart is a widely used model to simulate myocardial infarction and ischemia–reperfusion injury. Here we describe a ligation technique routinely performed in our laboratory to induce myocardial infarction that may be used to study ischemia–reperfusion injury in the myocardium. The methods described enhance location of the LAD coronary artery to allow for accurate ligation, thus increasing reproducibility of infarct size and location.

Key words

Myocardial ischemia–reperfusion Left anterior descending coronary artery Myocardial infarction LAD ligation Permanent ligation 


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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Zhaobin Xu
    • 1
  • Kevin E. McElhanon
    • 1
  • Eric X. Beck
    • 1
  • Noah Weisleder
    • 1
    Email author
  1. 1.Department of Physiology and Cell Biology, Davis Heart and Lung Research InstituteOhio State University Wexner Medical CenterColumbusUSA

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