A Murine Model of Hind Limb Ischemia to Study Angiogenesis and Arteriogenesis

  • Jun Yu
  • Alan DardikEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1717)


Therapeutic angiogenesis offers promise as a novel treatment that is complementary to surgical or endovascular procedures for peripheral arterial diseases (PAD). Appropriate development and use of hind limb ischemia models is necessary for successful studies of therapeutic angiogenesis and/or arteriogenesis. In this chapter, we describe two commonly used murine unilateral hind limb ischemia models, the femoral artery transection model and the femoral/saphenous artery excision model.

Key words

Hind limb ischemia Animal model Angiogenesis Arteriogenesis Ischemia Peripheral arterial disease 



This work was supported by NIH grants R01HL126933 and R56HL117064 and a grant from American Heart Association 14GRNT20450093 to JY; and NIH grants R01-HL095498 and R56-HL095498 as well as the United States Department of Veterans Affairs Biomedical Laboratory Research and Development Program Merit Review Award I01-BX002336 to AD.


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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Yale University School of MedicineNew HavenUSA

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