Mouse Injury Model of Polytrauma and Shock
Severe injury and shock remain major sources of morbidity and mortality worldwide. Immunologic dysregulation following trauma contributes to these poor outcomes. Few, if any, therapeutic interventions have benefited these patients, and this is due to our limited understanding of the host response to injury and shock. The Food and Drug Administration requires preclinical animal studies prior to any interventional trials in humans; thus, animal models of injury and shock will remain the mainstay for trauma research. However, adequate animal models that reflect the severe response to trauma in both the acute and subacute phases have been limited. Here we describe a novel murine model of polytrauma and shock that combines hemorrhagic shock, cecectomy, long bone fracture, and soft-tissue damage. This model produces an equivalent Injury Severity Score associated with adverse outcomes in humans, and may better recapitulate the human leukocyte, cytokine, transcriptomic, and overall inflammatory response following injury and hemorrhagic shock.
Key wordsMouse Polytrauma Hemorrhage Inflammation Immunity
J.C.M., T.L.M., and B.M. were supported by a training grant in burn and trauma research from the National Institute of General Medical Sciences (NIGMS) (T32 GM-008721). This work was also supported by NIH Grants R01 GM-040586 and R01 GM-081923, awarded by the NIGMS. A.M.M. was supported by R01 GM-105893. In addition, P.A.E. was supported by P30 AG-028740 from the NIH National Institute on Aging and R01 GM113945 (NIGMS). Finally, P.A.E. and L.L.M. were supported by P50 GM-111152 (NIGMS).
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