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Measuring Nascent Transcripts by Nascent-seq

  • Fei Xavier ChenEmail author
  • Stacy A. Marshall
  • Yu Deng
  • Sun Tianjiao
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1712)

Abstract

A complete understanding of transcription and co-transcriptional RNA processing events by polymerase requires precise and robust approaches to visualize polymerase progress and quantify nascent transcripts on a genome-wide scale. Here, we present a transcriptome-wide method to measure the level of nascent transcribing RNA in a fast and unbiased manner.

Key words

Nascent-seq Transcription Pol II mRNA Next-generation sequencing 

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Fei Xavier Chen
    • 1
    Email author
  • Stacy A. Marshall
    • 1
  • Yu Deng
    • 1
  • Sun Tianjiao
    • 1
  1. 1.Department of Biochemistry and Molecular Genetics, Feinberg School of MedicineNorthwestern UniversityChicagoUSA

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