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Investigate Global Chromosomal Interaction by Hi-C in Human Naive CD4 T Cells

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Next Generation Sequencing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1712))

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Abstract

Hi-C is a methodology developed to reveal chromosomal interactions from a genome-wide perspective. Here, we described a protocol for generating Hi-C sequencing libraries in resting and activated human naive CD4 T cells to investigate activation-induced chromatin structure re-arrangement in T cell activation followed by a section reviewing the general concepts of Hi-C data analysis.

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Acknowledgment

This is dedicated to the memory of Dr. Daniel R. Salomon. This work was supported by NIH grant 5U19 AI063603 and Mendez National Institute of Transplantation Foundation.

Author information

Authors and Affiliations

  1. Department of Molecular Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, SR-111, La Jolla, CA, 92037, USA

    Xiangzhi Meng

  2. Department of Inflammation Biology, La Jolla Institute of Allergy and Immunology, La Jolla, CA, USA

    Nicole Riley

  3. Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, USA

    Ryan Thompson

  4. Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, USA

    Siddhartha Sharma

Authors
  1. Xiangzhi Meng
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  2. Nicole Riley
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  3. Ryan Thompson
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  4. Siddhartha Sharma
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Corresponding author

Correspondence to Xiangzhi Meng .

Editor information

Editors and Affiliations

  1. Next Generation Sequencing and Microarray Core Facility, The Scripps Research Institute, La Jolla, California, USA

    Steven R. Head

  2. Next Generation Sequencing and Microarray Core Facility, The Scripps Research Institute, La Jolla, California, USA

    Phillip Ordoukhanian

  3. Department of Molecular Medicine, The Scripps Research Institute Center for Organ and Cell Transplantation, La Jolla, California, USA

    Daniel R. Salomon

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Meng, X., Riley, N., Thompson, R., Sharma, S. (2018). Investigate Global Chromosomal Interaction by Hi-C in Human Naive CD4 T Cells. In: Head, S., Ordoukhanian, P., Salomon, D. (eds) Next Generation Sequencing. Methods in Molecular Biology, vol 1712. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7514-3_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7514-3_15

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7512-9

  • Online ISBN: 978-1-4939-7514-3

  • eBook Packages: Springer Protocols

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