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Gene Profiling and T Cell Receptor Sequencing from Antigen-Specific CD4 T Cells

  • Marie Holt
  • Anne Costanzo
  • Louis Gioia
  • Brian Abe
  • Andrew I. Su
  • Luc TeytonEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1712)

Abstract

The paucity of pathogenic T cells in circulating blood limits the information delivered by bulk analysis. Toward diagnosis and monitoring of treatments of autoimmune diseases, we have devised single-cell analysis approaches capable of identifying and characterizing rare circulating CD4 T cells.

Key words

CD4 T cells Single-cell analysis TCR sequences Autoimmunity Peripheral blood Microfluidics 

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Marie Holt
    • 1
  • Anne Costanzo
    • 1
  • Louis Gioia
    • 2
  • Brian Abe
    • 1
  • Andrew I. Su
    • 2
  • Luc Teyton
    • 1
    Email author
  1. 1.Department of Immunology and MicrobiologyThe Scripps Research InstituteLa JollaUSA
  2. 2.Department of Integrative Structural and Computational BiologyThe Scripps Research InstituteLa JollaUSA

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