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An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome

  • Hyun Yong Jin
  • Changchun XiaoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1712)

Abstract

Recent advances in global translatome analysis technologies enable us to understand how translational regulation of gene expression modulates cellular functions. In this chapter, we present an integrated method to measure various aspects of translatome by polysome profiling and ribosome profiling using purified B cells. We standardized our protocols to directly compare the results from these two approaches. Parallel assessment of translatome with these two approaches can generate a comprehensive picture on how translational regulation determines protein output.

Key words

Translatome Ribosome profiling Ribo-seq Polysome profiling Translational regulation of gene expression 

Notes

Acknowledgments

We thank Jovan Shepherd for critical reading of manuscript. C.X. is a Pew Scholar in Biomedical Sciences. This study is supported by the PEW Charitable Trusts, Cancer Research Institute, National Institute of Health (R01AI087634, R01AI089854, RC1CA146299, R56AI110403, and R01AI121155 to C.X.).

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Department of Immunology and Microbial ScienceThe Scripps Research InstituteLa JollaUSA

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