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Using a Next-Generation Sequencing Approach to Profile MicroRNAs from Human Origin

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Preeclampsia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1710))

Abstract

Next-generation sequencing is a powerful method to interrogate the nucleotide composition for millions of DNA strands simultaneously. This technology can be utilized to profile microRNAs from multiple origins, such as tissues, cells, and body fluids. Next-generation sequencing is increasingly becoming a common and readily available technique for all laboratories. However, the bottleneck for next-generation sequencing is not within the laboratory but with the bioinformatics and data analysis of next-generation sequencing data. This chapter briefly describes the methods used to prepare samples for next-generation sequencing within the laboratory, before a deeper description of the methods used for data analysis.

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Acknowledgment

CS was in receipt of a Lions Medical Research Foundation Fellowship. This study was supported by the Lions Medical Research Foundation, UQ ECR Award, Royal Brisbane and Women’s Foundation, Diabetes Australia, and UQ-Ochsner Seed Grant. The ISO17025 accredited research facility was supported by grants from the Therapeutics Innovation Australia and the National Collaborative Research Infrastructure Strategy.

This review is supported partly by funding from the Lions Medical Research Foundation (LMRF), The University of Queensland, and Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT 1170809), Chile.

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Authors and Affiliations

  1. Exosome Biology Laboratory, Centre for Clinical Diagnostics, University of Queensland Centre for Clinical Research, Royal Brisbane and Women’s Hospital, The University of Queensland, Brisbane, QLD, 4029, Australia

    Dominic Guanzon, Juvita Delancy Iljas, Gregory E. Rice & Carlos Salomon

  2. Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Ochsner Clinic Foundation, New Orleans, LA, USA

    Gregory E. Rice & Carlos Salomon

  3. Department of Clinical Biochemistry and Immunology, Faculty of Pharmacy, University of Concepción, Concepción, Chile

    Carlos Salomon

  4. Faculty of Health Sciences, Center for Clinical Diagnostics, UQ Centre for Clinical Research, The University of Queensland, Bldg. 71/918, Royal Brisbane Hospital, Herston, QLD, 4029, Australia

    Carlos Salomon

Authors
  1. Dominic Guanzon
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  2. Juvita Delancy Iljas
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  3. Gregory E. Rice
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  4. Carlos Salomon
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Corresponding author

Correspondence to Carlos Salomon .

Editor information

Editors and Affiliations

  1. Monash Medical Centre, Monash University, Clayton, Victoria, Australia

    Padma Murthi

  2. INRS-Institut Armand-Frappier, Laval, Québec, Canada

    Cathy Vaillancourt

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Guanzon, D., Iljas, J.D., Rice, G.E., Salomon, C. (2018). Using a Next-Generation Sequencing Approach to Profile MicroRNAs from Human Origin. In: Murthi, P., Vaillancourt, C. (eds) Preeclampsia . Methods in Molecular Biology, vol 1710. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7498-6_16

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  • DOI: https://doi.org/10.1007/978-1-4939-7498-6_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7497-9

  • Online ISBN: 978-1-4939-7498-6

  • eBook Packages: Springer Protocols

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