Post-Bisulfite Adaptor Tagging for PCR-Free Whole-Genome Bisulfite Sequencing
Post-bisulfite adaptor tagging (PBAT) is a highly efficient procedure to construct libraries for whole-genome bisulfite sequencing (WGBS). PBAT attaches adaptors to bisulfite-converted genomic DNA to circumvent bisulfite-induced degradation of library DNA inherent to conventional WGBS protocols. Consequently, it enables PCR-free WGBS from nanogram quantities of mammalian DNA, thereby serving as an invaluable tool for methylomics.
Key wordsMethylome Next-generation sequencing PCR-free Single-nucleotide resolution
This work was supported by Research Program of Innovative Cell Biology by Innovative Technology (Cell Innovation) of the Ministry of Education, Culture, Sports, Science and Technology (MEXT) and the Platform Project for Supporting in Drug Discovery and Life Science Research (Platform for Drug Discovery, Informatics and Structural Life Science) of AMED.