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Post-Bisulfite Adaptor Tagging for PCR-Free Whole-Genome Bisulfite Sequencing

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1708)

Abstract

Post-bisulfite adaptor tagging (PBAT) is a highly efficient procedure to construct libraries for whole-genome bisulfite sequencing (WGBS). PBAT attaches adaptors to bisulfite-converted genomic DNA to circumvent bisulfite-induced degradation of library DNA inherent to conventional WGBS protocols. Consequently, it enables PCR-free WGBS from nanogram quantities of mammalian DNA, thereby serving as an invaluable tool for methylomics.

Key words

Methylome Next-generation sequencing PCR-free Single-nucleotide resolution 

Notes

Acknowledgments

This work was supported by Research Program of Innovative Cell Biology by Innovative Technology (Cell Innovation) of the Ministry of Education, Culture, Sports, Science and Technology (MEXT) and the Platform Project for Supporting in Drug Discovery and Life Science Research (Platform for Drug Discovery, Informatics and Structural Life Science) of AMED.

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Department of BiochemistryKyushu University Graduate School of Medical SciencesFukuokaJapan
  2. 2.Core Research for Evolutional Science and Technology (CREST)Japan Agency for Medical Research and Development (AMED)FukuokaJapan
  3. 3.Precursory Research for Embryonic Science and Technology (PRESTO)Japan Science and Technology Agency (JST)FukuokaJapan

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