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Methyl-CpG-Binding Domain Sequencing: MBD-seq

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DNA Methylation Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1708))

Abstract

Detailed biological knowledge about the potential importance of the methylome is typically lacking for common diseases. Therefore, methylome-wide association studies (MWAS) are critical to detect disease relevant methylation sites. Methyl-CpG-binding domain sequencing (MBD-seq) offers potential advantages compared to antibody-based enrichment, but performance depends critically on using an optimal protocol. Using an optimized protocol, MBD-seq can approximate the sensitivity/specificity obtained with whole-genome bisulfite sequencing, but at a fraction of the costs and time to complete the project. Thus, MBD-seq offers a comprehensive first pass at the CpG methylome and is economically feasible with the samples sizes required for MWAS.

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Author information

Authors and Affiliations

  1. Center for Biomarker Research and Precision Medicine, Virginia Commonwealth University, P. O. Box 980533, Richmond, VA, 23298, USA

    Karolina A. Aberg, Robin F. Chan, Linying Xie, Andrey A. Shabalin & Edwin J. C. G. van den Oord

Authors
  1. Karolina A. Aberg
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  2. Robin F. Chan
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  3. Linying Xie
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  4. Andrey A. Shabalin
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  5. Edwin J. C. G. van den Oord
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Corresponding author

Correspondence to Edwin J. C. G. van den Oord .

Editor information

Editors and Affiliations

  1. Laboratory for Epigenetics and Environment, Centre National de Recherche en Génomique Humaine, CEA—Institut de Biologie Francois Jacob, Evry, France

    Jörg Tost

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Aberg, K.A., Chan, R.F., Xie, L., Shabalin, A.A., van den Oord, E.J.C.G. (2018). Methyl-CpG-Binding Domain Sequencing: MBD-seq. In: Tost, J. (eds) DNA Methylation Protocols. Methods in Molecular Biology, vol 1708. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7481-8_10

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  • DOI: https://doi.org/10.1007/978-1-4939-7481-8_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7479-5

  • Online ISBN: 978-1-4939-7481-8

  • eBook Packages: Springer Protocols

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