Abstract
Heat shock transcription factors (Hsfs) regulate transcription of heat shock proteins as well as other genes whose promoters contain heat shock elements (HSEs). There are at least five Hsfs in mammalian cells, Hsf1, Hsf2, Hsf3, Hsf4, and Hsfy (Wu, Annu Rev Cell Dev Biol 11:441–469, 1995; Morimoto, Genes Dev 12:3788–3796, 1998; Tessari et al., Mol Hum Repord 4:253–258, 2004; Fujimoto et al., Mol Biol Cell 21:106–116, 2010; Nakai et al., Mol Cell Biol 17:469–481, 1997; Sarge et al., Genes Dev 5:1902–1911, 1991). To understand the physiological roles of Hsf1, Hsf2, and Hsf4 in vivo, we generated knockout mouse lines for these factors (Zhang et al., J Cell Biochem 86:376–393, 2002; Wang et al., Genesis 36:48–61, 2003; Min et al., Genesis 40:205–217, 2004). Numbers of other laboratories have also generated Hsf1 (Xiao et al., EMBO J 18:5943–5952, 1999; Sugahara et al., Hear Res 182:88–96, 2003), Hsf2 (McMillan et al., Mol Cell Biol 22:8005–8014, 2002; Kallio et al., EMBO J 21:2591–2601, 2002), and Hsf4 (Fujimoto et al., EMBO J 23:4297–4306, 2004) knockout mouse models. In this chapter, we describe the design of the targeting vectors, the plasmids used, and the successful generation of mice lacking the individual genes. We also briefly describe what we have learned about the physiological functions of these genes in vivo.
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Acknowledgements
This work was supported by VA Award 1I01BX000161 and NIH grants CA062130 and CA132640 (N.F.M.) and CA121951 and CA121951-07S2 (D.M.). For generation of hsf knockout mice, the microinjection of ES cells and generation of chimeras were conducted in the Medical College of Georgia (Augusta University) Embryonic Stem Cell and Transgenic Core Facility.
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Jin, X., Eroglu, B., Moskophidis, D., Mivechi, N.F. (2018). Targeted Deletion of Hsf1, 2, and 4 Genes in Mice. In: Calderwood, S., Prince, T. (eds) Chaperones. Methods in Molecular Biology, vol 1709. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7477-1_1
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DOI: https://doi.org/10.1007/978-1-4939-7477-1_1
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