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Single-Particle Tracking of Cell Surface Proteins

  • Laabiah Wasim
  • Bebhinn TreanorEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1707)

Abstract

Single-particle tracking has been used extensively to advance our understanding of the plasma membrane and the mechanisms controlling the movement of cell surface proteins. These studies provide fundamental insights into the regulation of membrane receptor activation and the assembly of signaling clusters. Here, we describe a method to label and track B cell receptor (BCR) and other cell surface proteins and how this method can be adapted to simultaneously track two molecular species or examine the movement of membrane proteins in relation to membrane microdomains. We recently used this method to study the role of the actin cytoskeleton in the regulation of B cell receptor dynamics at the cell surface.

Key words

Single-particle tracking SPT Membrane proteins Protein dynamics 

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Department of ImmunologyUniversity of TorontoTorontoCanada
  2. 2.Department of Cell and Systems BiologyUniversity of TorontoTorontoCanada
  3. 3.Department of Biological SciencesUniversity of Toronto ScarboroughTorontoCanada

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