RNA Interference to Knock Down Gene Expression

  • Haiyong HanEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1706)


RNA interference (RNAi) is a biological process by which double-stranded RNA (dsRNA) induces sequence-specific gene silencing by targeting mRNA for degradation. As a tool for knocking down the expression of individual genes posttranscriptionally, RNAi has been widely used to study the cellular function of genes. In this chapter, I describe procedures for using gene-specific, synthetic, short interfering RNA (siRNA) to induce gene silencing in mammalian cells. Protocols for using lipid-based transfection reagents and electroporation techniques are provided. Potential challenges and problems associated with the siRNA technology are also discussed.

Key words

RNA interference RNAi siRNA Gene silencing Transfection Electroporation 



This work was supported by NIH/NCI grants CA169281 and CA191923 to H.H.


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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Translational Genomics Research InstitutePhoenixUSA

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