Study of Plasmid-Mediated Quinolone Resistance in Bacteria

  • George A. JacobyEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1703)


Plasmid-mediated quinolone resistance (PMQR) involves genes for proteins that protect the quinolone targets, an enzyme that inactivates certain quinolones as well as aminoglycosides, and pumps that efflux quinolones. Quinolone susceptibility is reduced by these mechanisms but not to the level of clinical resistance unless chromosomal mutations are also present. PCR primers and conditions for PMQR gene detection are described as well as how to establish a plasmid location.

Key words

Quinolone resistance Conjugation Gene location Plasmid characterization 


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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Lahey Hospital and Medical CenterBurlingtonUSA

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