Large-Scale Femtoliter Droplet Array for Single Cell Efflux Assay of Bacteria

  • Ryota Iino
  • Shouichi Sakakihara
  • Yoshimi Matsumoto
  • Kunihiko Nishino
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1700)

Abstract

Large-scale femtoliter droplet array as a platform for single cell efflux assay of bacteria is described. Device microfabrication, femtoliter droplet array formation and concomitant enclosure of single bacterial cells, fluorescence-based detection of efflux activity at the single cell level, and collection of single cells from droplet and subsequent gene analysis are described in detail.

Key words

Microfabrication Microdroplet array Single cell analysis Drug efflux AcrAB-TolC MexAB-OprM Escherichia coli Salmonella enterica Pseudomonas aeruginosa Optical microscopy Fluorescence 

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Ryota Iino
    • 1
    • 2
  • Shouichi Sakakihara
    • 3
  • Yoshimi Matsumoto
    • 4
  • Kunihiko Nishino
    • 5
    • 6
  1. 1.Okazaki Institute for Integrative Bioscience, Institute for Molecular Science, National Institutes of Natural SciencesOkazakiJapan
  2. 2.Department of Functional Molecular ScienceSchool of Physical Sciences, The Graduate University for Advanced Studies (SOKENDAI)KanagawaJapan
  3. 3.Technical DivisionInstitute of Scientific and Industrial Research, Osaka UniversityOsakaJapan
  4. 4.Department of Biomolecular Science and RegulationInstitute of Scientific and Industrial Research, Osaka UniversityIbarakiJapan
  5. 5.Institute of Scientific and Industrial Research, Osaka UniversityIbarakiJapan
  6. 6.Graduate School of Pharmaceutical Sciences, Osaka UniversitySuitaJapan

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