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Construction of Bovine Immunoglobulin Libraries in the Single-Chain Fragment Variable (scFv) Format

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Phage Display

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1701))

Abstract

Recombinant immunoglobulins are an excellent tool for diagnosis, treatment, and passive immunization. Phage display offers a robust technique for the selection of recombinant antibodies from immunoglobulin libraries. The construction of immunoglobulin libraries for veterinary purposes was restricted by the lack of knowledge about species-specific diversities.

The now available data enable the construction of highly diverse libraries in livestock like cattle. Using diverse primer sets, the immunoglobulin repertoire is amplified and ligated into a phagemid. Infection of E. coli with filamentous phages allows the display of the immunoglobulin fragments on the surface as a fusion protein to the phage’s minor coat protein 3.

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Acknowledgments

I gratefully thank Dr. Apostolos Gittis and Dr. David Garboczi (both Structural Biology Section, Research Technologies Branch, NIAID, NIH, Maryland, USA) for critical review of the manuscript.

This material should not be interpreted as representing the viewpoint of the U.S. Department of Health and Human Services, the National Institutes of Health, or the Laboratory of Viral Diseases-Genetic Engineering Section of the National Institute of Allergy and Infectious Diseases.

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Authors and Affiliations

  1. Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 33 North Drive, Bethesda, MD, 20892, USA

    Ulrike S. Diesterbeck

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  1. Ulrike S. Diesterbeck
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Correspondence to Ulrike S. Diesterbeck .

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Editors and Affiliations

  1. Technische Universität Braunschweig, Braunschweig, Germany

    Michael Hust

  2. Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden, Penang, Malaysia

    Theam Soon Lim

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Diesterbeck, U.S. (2018). Construction of Bovine Immunoglobulin Libraries in the Single-Chain Fragment Variable (scFv) Format. In: Hust, M., Lim, T. (eds) Phage Display. Methods in Molecular Biology, vol 1701. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7447-4_6

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  • DOI: https://doi.org/10.1007/978-1-4939-7447-4_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7446-7

  • Online ISBN: 978-1-4939-7447-4

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