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MicroRNA Sequencing Data Analysis Toolkits

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1699)

Abstract

MicroRNA (miRNA) is a non-protein-coding small RNA molecule that negatively regulates gene expression by degradation of mRNA or suppression of mRNA translation. MiRNAs play important roles in biological processes such as cellular development, differentiation, proliferation, apoptosis and stem cell self-renewal and cancer development. The expression profile of microRNAs is tissue-, cell-type specific. PCR- and microarray-based arrays are the commonly used for differential expression of microRNAs between different diseased conditions. With the next-generation sequencing or massively parallel DNA sequencing technology advanced and the cost is plummeting, microRNAseq approach is widely used for the genome-wide discovery of known and unknown miRNA expression. However, the analysis of microRNAseq is computational expensive, here I provide guidelines and available tools for microRNAseq analysis.

Key words

microRNA microRNAseq Massively parallel DNA sequencing Bioinformatics Data analysis 

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Department of Medicine, Helen Diller Family Comprehensive Cancer CenterUniversity of California in San FranciscoSan FranciscoUSA

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