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Optogenetics: A Roadmap pp 89–116Cite as

Optogenetic Applications in the Nematode Caenorhabditis elegans

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Part of the book series: Neuromethods ((NM,volume 133))

Abstract

The advantages of the nematode Caenorhabditis elegans, such as a well-characterized nervous system, complex behavioral patterns, powerful genetics, and experimental tractability, establish this animal as an excellent platform for optogenetic studies and manipulation. A roadmap for conducting optogenetic experiments in C. elegans is provided in this chapter. We give advice on the choice of appropriate optogenetic tools, generation of transgenic animals and the preparation of animals for experiments, and describe using the nematode for optogenetic tool engineering by detecting body wall muscle contraction. We also survey specific optogenetic applications in C. elegans that give insight in long-term behavior and development; all-optical interrogation, combining optogenetic neuronal manipulation with the simultaneous detection of neural activity by calcium sensors; the optogenetic generation of reactive oxygen species for cell and protein ablation and mutagenesis; optogenetic control of intracellular signaling pathways; and the harnessing of optogenetics for a drug-screening platform by pacing pharyngeal muscle contraction.

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Acknowledgments

We thank the Wellcome Trust (109614/Z/15/Z) and the Medical Research Council (MR/N004574/1) for financial support.

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Authors and Affiliations

  1. Centre for Discovery Brain Sciences, Edinburgh Medical School: Biomedical Sciences, University of Edinburgh, Hugh Robson Bldg., George Square, Edinburgh, EH8 9XD, Scotland

    Katharina Elisabeth Fischer, Nathalie Alexandra Vladis & Karl Emanuel Busch

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  1. Katharina Elisabeth Fischer
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  2. Nathalie Alexandra Vladis
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  3. Karl Emanuel Busch
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Correspondence to Karl Emanuel Busch .

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  1. Focus Program Translational Neurosciences and Institute for Microscopic Anatomy and Neurobiology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany

    Albrecht Stroh

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Fischer, K.E., Vladis, N.A., Busch, K.E. (2018). Optogenetic Applications in the Nematode Caenorhabditis elegans . In: Stroh, A. (eds) Optogenetics: A Roadmap. Neuromethods, vol 133. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7417-7_6

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