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Ocular Hypertension/Glaucoma in Minipigs: Episcleral Veins Cauterization and Microbead Occlusion Methods

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Glaucoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1695))

Abstract

Two methods to induce elevation of the intraocular pressure (experimental glaucoma) are described in the present chapter. The first method is based on increasing the post-trabecular resistance to aqueous outflow by cauterizing the episcleral veins (EVC). This method allows the observation of ultrastructural changes in the trabecular meshwork (TM) without interfering with any structure within the eye such as TM, ciliary body, and/or the Retina. The second method is the multiple injection of microbeads into the anterior chamber, as a pre and intra-trabecular method that induce secondary effects on the TM cells. Both methods lead to an increase in IOP.

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Acknowledgments

We want to specially thank Francisco Martin for his suggestions to improve the method and handling of animals. During the development of the methods a grant from The Glaucoma Foundation (TGF) supported the research.

Author information

Authors and Affiliations

  1. Experimental Ophathalmo-Biology Group, Department Cell Biology and Histology, University of the Basque Country, Barrio Sarriena s/n, Leioa, 48940, Vizcaya, Spain

    Elena Vecino & Haritz Urcola

  2. Ophthalmology Section, Hospital Universitario Araba, Vitoria, Spain

    Haritz Urcola

  3. Department of Veterinary Ophthalmology, Murcia University, Murcia, Spain

    Alejando Bayon

  4. Department of Anatomy and Cell Biology, New York Medical College, Valhalla, NY, USA

    Sansar C. Sharma

Authors
  1. Elena Vecino
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  2. Haritz Urcola
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  3. Alejando Bayon
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  4. Sansar C. Sharma
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Corresponding author

Correspondence to Elena Vecino .

Editor information

Editors and Affiliations

  1. Department of Ophthalmology, Massachusetts Eye and Ear Infirmary/Schepens Eye, Research Institute, Harvard Medical School, Boston, Massachusetts, USA

    Tatjana C. Jakobs

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Vecino, E., Urcola, H., Bayon, A., Sharma, S.C. (2018). Ocular Hypertension/Glaucoma in Minipigs: Episcleral Veins Cauterization and Microbead Occlusion Methods. In: Jakobs, T. (eds) Glaucoma. Methods in Molecular Biology, vol 1695. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7407-8_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7407-8_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7406-1

  • Online ISBN: 978-1-4939-7407-8

  • eBook Packages: Springer Protocols

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