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Cancer Stem Cells pp 1–16Cite as

Introduction to Cancer Stem Cells: Past, Present, and Future

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1692))

Abstract

The Cancer Stem Cell (CSC) hypothesis postulates the existence of a small population of cancer cells with intrinsic properties allowing for resistance to conventional radiochemotherapy regiments and increased metastatic potential. Clinically, the aggressive nature of CSCs has been shown to correlate with increased tumor recurrence, metastatic spread, and overall poor patient outcome across multiple cancer subtypes. Traditionally, isolation of CSCs has been achieved through utilization of cell surface markers, while the functional differences between CSCs and remaining tumor cells have been described through proliferation, differentiation, and limiting dilution assays. The generated insights into CSC biology have further highlighted the importance of studying intratumoral heterogeneity through advanced functional assays, including CRISPR-Cas9 screens in the search of novel targeted therapies. In this chapter, we review the discovery and characterization of cancer stem cells populations within several major cancer subtypes, recent developments of novel assays used in studying therapy resistant tumor cells, as well as recent developments in therapies targeted at cancer stem cells.

The original version of this chapter was revised. An erratum to this chapter can be found at DOI 10.1007/978-1-4939-7401-6_19

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Authors and Affiliations

  1. McMaster Stem Cell and Cancer Research Institute, McMaster University, Hamilton, ON, Canada, L8S 4K1

    David Bakhshinyan, Ashley A. Adile, Maleeha A. Qazi, Mohini Singh, Michelle M. Kameda-Smith, Nick Yelle, Chirayu Chokshi, Chitra Venugopal & Sheila K. Singh

  2. Department of Biochemistry and Biomedical Science, Faculty of Health Sciences, McMaster University, Hamilton, ON, Canada, L8S 4K1

    David Bakhshinyan, Ashley A. Adile, Maleeha A. Qazi, Mohini Singh, Michelle M. Kameda-Smith, Nick Yelle, Chirayu Chokshi & Sheila K. Singh

  3. Department of Surgery, Faculty of Health Sciences, McMaster University, Hamilton, ON, Canada, L8S 4K1

    Chitra Venugopal & Sheila K. Singh

  4. Michael DeGroote Centre for Learning and Discovery, Stem Cell and Cancer Research Institute, McMaster University, MDCL 5027, 1280 Main Street West, Hamilton, ON, Canada, L8S 4K1

    Sheila K. Singh

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  1. David Bakhshinyan
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  2. Ashley A. Adile
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  3. Maleeha A. Qazi
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  4. Mohini Singh
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  5. Michelle M. Kameda-Smith
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  6. Nick Yelle
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  7. Chirayu Chokshi
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  8. Chitra Venugopal
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  9. Sheila K. Singh
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Correspondence to Sheila K. Singh .

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Editors and Affiliations

  1. Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples, Italy

    Gianpaolo Papaccio

  2. Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples, Italy

    Vincenzo Desiderio

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Bakhshinyan, D. et al. (2018). Introduction to Cancer Stem Cells: Past, Present, and Future. In: Papaccio, G., Desiderio, V. (eds) Cancer Stem Cells. Methods in Molecular Biology, vol 1692. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7401-6_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7401-6_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7400-9

  • Online ISBN: 978-1-4939-7401-6

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