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Detection of Thermospermine and Spermine by HPLC in Plants

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Polyamines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1694))

Abstract

Thermospermine, a structural isomer of spermine, is widely spread in the plant kingdom and has recently been shown to play a key role in the repression of xylem differentiation in vascular plants. However, a standard high-performance liquid chromatography (HPLC) protocol for detecting polyamines as their dansyl derivative cannot distinguish themospermine from spermine. These isomers become separated from each other after benzoylation. In this chapter, we describe a simple protocol for extraction, benzoylation, and HPLC detection of thermospermine and spermine with other polyamines from plant material.

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References

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Acknowledgment

This work was supported in part by Grants-in-Aid for Scientific Research [No. 16H0124518] from the Japan Society for the Promotion of Science (JSPS) to T.T.

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Authors and Affiliations

  1. Division of Earth, Life, and Molecular Sciences, Graduate School of Natural Science and Technology, Okayama University, Tsushimanaka 3-1-1, kita-ku, 700-8530, Okayama, Japan

    Taku Takahashi, Ayaka Takano & Jun-Ichi Kakehi

Authors
  1. Taku Takahashi
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  2. Ayaka Takano
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  3. Jun-Ichi Kakehi
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Corresponding author

Correspondence to Taku Takahashi .

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Editors and Affiliations

  1. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Rubén Alcázar

  2. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Antonio F. Tiburcio

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Takahashi, T., Takano, A., Kakehi, JI. (2018). Detection of Thermospermine and Spermine by HPLC in Plants. In: Alcázar, R., Tiburcio, A. (eds) Polyamines. Methods in Molecular Biology, vol 1694. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7398-9_6

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  • DOI: https://doi.org/10.1007/978-1-4939-7398-9_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7397-2

  • Online ISBN: 978-1-4939-7398-9

  • eBook Packages: Springer Protocols

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