Polyamines pp 373-388 | Cite as

High-Throughput Phenotyping in Plant Stress Response: Methods and Potential Applications to Polyamine Field

  • D. Marko
  • N. Briglia
  • S. Summerer
  • A. Petrozza
  • F. Cellini
  • R. Iannacone
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1694)

Abstract

High-throughput phenotyping has opened whole new perspectives for crop improvement and better understanding of quantitative traits in plants. Generation of loss-of-function and gain-of-function plant mutants requires processing and imaging a large number of plants in order to determine unknown gene functions and phenotypic changes generated by genetic modifications or selection of new traits. The use of phenomics for the evaluation of transgenic lines contributed significantly to the identification of plants more tolerant to biotic/abiotic stresses and furthermore, helped in the identification of unknown gene functions. In this chapter we describe the High-throughput phenotyping (HTP) platform working in our facility, drawing the general protocol and showing some examples of data obtainable from the platform. Tomato transgenic plants over-expressing the arginine decarboxylase 2 gene, which is involved in the polyamine biosynthetic pathway, were analyzed through our HTP facility for their tolerance to abiotic stress and significant differences in water content and ability to recover after drought stress where highlighted. This demonstrates the applicability of this methodology to the plant polyamine field.

Key words

High-throughput phenotyping Abiotic stress Polyamines 3D Scanalyzer Platform Tomato 

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • D. Marko
    • 1
  • N. Briglia
    • 2
  • S. Summerer
    • 1
  • A. Petrozza
    • 1
  • F. Cellini
    • 1
  • R. Iannacone
    • 1
  1. 1.ALSIA—Metapontum Agrobios Research CenterMetaponto (MT)Italy
  2. 2.UNIBAS—Dipartimento delle Culture Europee e del Mediterraneo: Architettura, Ambiente, Patrimoni CulturaliMateraItaly

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