Abstract
In all cases when a bacteriophage makes direct contact with a mammalian organism, it may challenge the mammalian immunological system. Its major consequence is production of antibodies specific to the bacteriophage. Here we present protocols applicable in studies of bacteriophage ability to induce specific antibodies. The protocols have been divided into three parts: purification, immunization, and detection (ELISA).
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Acknowledgment
This work was supported by the National Science Centre in Poland, grant UMO-2013/08/M/NZ6/01022. Special thanks to Weronika Dąbrowska (Wrocław, Poland) for her artwork contribution (Fig. 1).
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Dąbrowska, K. (2018). Interaction of Bacteriophages with the Immune System: Induction of Bacteriophage-Specific Antibodies. In: Azeredo, J., Sillankorva, S. (eds) Bacteriophage Therapy. Methods in Molecular Biology, vol 1693. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7395-8_12
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DOI: https://doi.org/10.1007/978-1-4939-7395-8_12
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7395-8
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