Long-Term Imaging of Endoplasmic Reticulum Morphology in Embryos During Seed Germination

  • Natasha Dzimitrowicz
  • Emily Breeze
  • Lorenzo Frigerio
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1691)

Abstract

Imaging plant embryos at the cellular level over time is technically challenging, since the embryo, once its protective seed coat is removed, must be kept viable and unstressed on a microscope slide for the duration of the experiment. Here we describe a procedure and suitable apparatus for the visualization, over several days, of changes in endoplasmic reticulum (ER) morphology associated with the process of germination in Arabidopsis thaliana seeds. Moreover, we also present a user-friendly image analysis tool which enables subtle perturbations in the ER network to be measured.

Key words

Confocal microscopy Imaging chamber Embryo Germination 

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Natasha Dzimitrowicz
    • 1
  • Emily Breeze
    • 1
  • Lorenzo Frigerio
    • 1
  1. 1.School of Life SciencesUniversity of WarwickCoventryUK

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