Metabolons on the Plant ER

  • Verena KriechbaumerEmail author
  • Stanley W. Botchway
Part of the Methods in Molecular Biology book series (MIMB, volume 1691)


Metabolons are protein complexes that contain all the enzymes necessary for a metabolic pathway but also scaffolding proteins. Such a structure allows efficient channeling of intermediate metabolites from one active site to the next and is highly advantageous for labile or toxic intermediates. Here we describe two methods currently used to identify metabolons via protein–protein interaction methodology: immunoprecipitations using GFP-Trap®_A beads to find novel interaction partners and potential metabolon components and FRET-FLIM to test for and quantify protein–protein interactions in planta.

Key words

Endoplasmic reticulum Metabolon Enzymes Protein complex Protein–protein interaction Co-immunoprecipitation GFP-trap FRET-FLIM 



This work was supported by the Science and Technology Facilities Council Program (grant no. 14230008) awarded to Prof. Chris Hawes. 


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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Department of Biological and Medical SciencesOxford Brookes UniversityOxfordUK
  2. 2.Central Laser FacilityScience and Technology Facilities Council (STFC) Rutherford Appleton LaboratoryDidcotUK

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