ER Membrane Lipid Composition and Metabolism: Lipidomic Analysis

  • Laetitia Fouillen
  • Lilly Maneta-Peyret
  • Patrick Moreau
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1691)

Abstract

Plant ER membranes are the major site of biosynthesis of several lipid families (phospholipids, sphingolipids, neutral lipids such as sterols and triacylglycerols). The structural diversity of lipids presents considerable challenges to comprehensive lipid analysis. This chapter will briefly review the various biosynthetic pathways and will detail several aspects of the lipid analysis: lipid extraction, handling, separation, detection, identification, and data presentation. The different tools/approaches used for lipid analysis will also be discussed in relation to the studies to be carried out on lipid metabolism and function.

Key words

Ceramides GC-FID GC-MS Glucosylceramides HPTLC LC-MS Long chain bases (LCB) Phospholipids Phytosterols Triacylglycerols 

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Laetitia Fouillen
    • 1
    • 2
  • Lilly Maneta-Peyret
    • 1
  • Patrick Moreau
    • 1
    • 3
  1. 1.CNRS-University of Bordeaux, UMR 5200 Membrane Biogenesis LaboratoryVillenave d’OrnonFrance
  2. 2.MetaboHub-Metabolome Facility of BordeauxFunctional Genomics CenterBordeauxFrance
  3. 3.Bordeaux Imaging Center, UMS 3420 CNRS, US004 INSERMUniversity of BordeauxBordeauxFrance

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