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Protein NMR pp 99–109Cite as

Spherical Nanoparticle Supported Lipid Bilayers: A Tool for Modeling Protein Interactions with Curved Membranes

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1688))

Abstract

Mechanistic studies of protein-membrane interactions can be complicated by the limitations of the membrane model system chosen. Many of these limitations can be overcome by using a spherical silica nanoparticle to support the membrane. In this chapter, we present a detailed protocol for the construction of spherical nanoparticle supported lipid bilayers (SSLBs), with discussion of methods to improve production.

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Acknowledgment

We thank Nissan Chemical Industries, Ltd. for 50 and 100 nm silicon beads. This work was supported by the National Institutes of Health NIGMS (R01GM105963 to F.T.) and the Four Diamonds Fund. This project also is funded, in part, under a grant with the Pennsylvania Department of Health using Tobacco CURE Funds. The Department specifically disclaims responsibility for any analyses, interpretations or conclusions.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, The Pennsylvania State University, Hershey, PA, 17033, USA

    Erin R. Tyndall & Fang Tian

Authors
  1. Erin R. Tyndall
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  2. Fang Tian
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Correspondence to Fang Tian .

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Editors and Affiliations

  1. Department of Chemistry and Biochemistry, The City College of New York, New York, New York, USA

    Ranajeet Ghose

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Tyndall, E.R., Tian, F. (2018). Spherical Nanoparticle Supported Lipid Bilayers: A Tool for Modeling Protein Interactions with Curved Membranes. In: Ghose, R. (eds) Protein NMR. Methods in Molecular Biology, vol 1688. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7386-6_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7386-6_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7385-9

  • Online ISBN: 978-1-4939-7386-6

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