Protein NMR pp 257-310 | Cite as

From Raw Data to Protein Backbone Chemical Shifts Using NMRFx Processing and NMRViewJ Analysis

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1688)

Abstract

Assignment of the chemical shifts of the backbone atoms (HN, N, CA, CB, and C) of proteins is often a prerequisite to using NMR information in the study of proteins. These chemical shifts and their perturbations are the basis for the analysis of protein dynamics, ligand binding, and backbone conformation. They are generally assigned prior to full side-chain assignments and the determination of the complete three-dimensional molecular structure. This chapter describes the use of two software packages, NMRFx Processor and NMRViewJ, in going from raw NMR data to backbone assignments. The step-by-step procedure describes processing of the data and the use of manual and automated features of the RunAbout tool in NMRViewJ to perform the assignments.

Key words

Nuclear magnetic resonance (NMR) Chemical shifts Backbone assignments NMRViewJ NMRFx Processor 

Notes

Acknowledgments

This work was supported in part by a grant from the National Institute of General Medical Sciences of the National Institutes of Health (P50 GM 103297 to B.A.J.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Structural Biology InitiativeCUNY Advanced Science Research CenterNew YorkUSA
  2. 2.One Moon Scientific, Inc.WestfieldUSA

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