Transition metal ions play important structural, regulatory, and catalytic roles in all biological systems by serving as cofactors for proteins. Due to their relatively low levels in the cell compared to abundant metal ions such as potassium and magnesium, transition metals are often considered micronutrients and referred to as trace elements. Manganese (Mn), iron (Fe), copper (Cu), and zinc (Zn) are the most prevalent transition metals in the Lyme disease spirochete Borrelia burgdorferi. Here, we describe a method for the accurate measurement of these trace elements in B. burgdorferi utilizing inductively coupled plasma-sector field mass spectrometry (ICP-SFMS).
Borrelia burgdorferiTrace element analysis Transition metal ions Inductively coupled plasma-sector field mass spectrometry High resolution-inductively coupled plasma-mass spectrometry
This is a preview of subscription content, log in to check access
Springer Nature is developing a new tool to find and evaluate Protocols. Learn more
This work is supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under the award number AI103173. We thank Michael V. Norgard for providing the wild-type 297 and the bmtA mutant strains of B. burgdorferi.
Bolann BJ, Rahil-Khazen R, Henriksen H, Isrenn R, Ulvik RJ (2007) Evaluation of methods for trace-element determination with emphasis on their usability in the clinical routine laboratory. Scand J Clin Lab Invest 67(4):353–366. doi:10.1080/00365510601095281CrossRefPubMedGoogle Scholar
Jakubowski N, Prohaska T, Vanhaecke F, Roos PH, Lindemann T (2011) Inductively coupled plasma- and glow discharge plasma-sector field mass spectrometry. J Anal At Spectrom 26(4):727–757. doi:10.1039/C0ja00007hCrossRefGoogle Scholar
Pollack RJ, Telford SR 3rd, Spielman A (1993) Standardization of medium for culturing Lyme disease spirochetes. J Clin Microbiol 31(5):1251–1255PubMedPubMedCentralGoogle Scholar