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Analysis of Lipids and Lipid Rafts in Borrelia

  • Alvaro Toledo
  • Zhen Huang
  • Jorge L. Benach
  • Erwin London
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1690)

Abstract

Lipid rafts are membrane microdomains that are involved in cellular processes such as protein trafficking and signaling processes, and which play a fundamental role in membrane fluidity and budding. The lipid composition of the membrane and the biochemical characteristics of the lipids found within rafts define the ability of cells to form microdomains and compartmentalize the membrane. In this chapter, we describe the biophysical, biochemical, and molecular approaches used to define and characterize lipid rafts in the Lyme disease agent, Borrelia burgdorferi.

Key words

Lipid rafts Borrelia Lyme disease Lipids 

Notes

Acknowledgments

This work was supported by NIH grants 1R21AI125806 to AT, GM 099892 to EL and RO1-AI-027044 to JLB.

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Alvaro Toledo
    • 1
  • Zhen Huang
    • 3
  • Jorge L. Benach
    • 2
  • Erwin London
    • 3
  1. 1.Department of EntomologyRutgers UniversityNew BrunswickUSA
  2. 2.Department of Molecular Genetics and MicrobiologyStony Brook UniversityStony BrookUSA
  3. 3.Department of Biochemistry and Cell BiologyStony Brook UniversityStony BrookUSA

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